Journal:

Article Title: Targeting Tryptophan Decarboxylase to Selected Subcellular Compartments of Tobacco Plants Affects Enzyme Stability and in Vivo Function and Leads to a Lesion-Mimic Phenotype 1

doi: 10.1104/pp.010889

Figure Lengend Snippet: Immunoblot analysis of the crude TSP extract of independent transgenic tobacco plants expressing targeted TDC to the chloroplast (A), cytosol (B), and ER (C). The 9E10 anti-c-myc antibody (A and B) or the MA-GRP78 anti-KDEL antibody (C) was used to detect the recombinant TDC. M, Prestained protein marker. Lanes 1 through 6A and lanes 1 through 8, B and C, Leaf crude extract of transgenic T1 (A and B) and T0 plants (C). W, TSP of wild-type tobacco SR1 plants.

Article Snippet: The primary 9E10 anti- c-myc antibody (clone no. CRL-1729; American Type Culture Collection, Manassas, VA) was used to detect the chloroplast- and cytosol-targeted TDC, whereas the mouse anti-GRP78 anti-KDEL antibody (StressGen Biotechnologies, Victoria, BC) was used to detect the ER-targeted TDC.

Techniques: Western Blot, Transgenic Assay, Expressing, Recombinant, Marker

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Mechanism of Bactericidal Activity of Microcin L in Escherichia coli and Salmonella enterica ▿

doi: 10.1128/AAC.01217-10

Figure Lengend Snippet: Antibacterial activity of MccL against wild-type and receptor mutant strains

Article Snippet: In conclusion, only the catecholate siderophore receptor Cir, from E. coli and Salmonella enterica , was identified as a receptor for MccL. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Parent strain Derived mutant Relevant receptor gene(s) Inhibition zone (mm) Escherichia coli AB2847 23 P8 a fhuA 23 MC4100 23 H1443 24 MS172 a fhuE 22 ZI311 a fecA 25 H1876 a fepA , fiu , cir 0 H873 a fepA 24 H1594 a fiu 23 H1877 a fepA , fiu 25 VR42 a cir 0 H1875 a fepA , cir 0 H1728 a fiu , cir 0 H1729 a fhuA , fiu , cir 0 DB503 16 AB4 b manY 15 AB3 b manXYZ 16 MC4100 16 MnTM b sdaC 18 Salmonella Typhimurium ATCC 14028 8 WR 1726 a fepA 6 AJB 64 a iroN 15 AR 8412 a cir 0 WR 1727 a fepA , iroN 10 WR 1728 a fepA , iroN , cir 0 Salmonella Enteritidis SE 147 Nal r 8 WR 1425 a fepA 11 WR 1434 a fepA , iroN 11 WR 1530 a cir 0 WR 1458 a iroN , cir 0 Open in a separate window a Strains with a mutated siderophore receptor of the outer membrane. b Strains with a mutated cytoplasmic membrane receptor.

Techniques: Activity Assay, Mutagenesis, Derivative Assay, Inhibition